Dr. Ruth Carmichael and Tina Schrader were featured in a video blog post from Promega, discussing research from the Schrader lab as well as how they have used Promega's Nano-Glo® Dual-Luciferase® Reporter (DLR) assay system together with the GloMax® Discover plate reader to measure endogenous expression of peroxisome proteins. Ruth and Tina used a CRISPR-Cas9 based strategy to endogenously tag peroxisomal proteins with the NanoLuc® luciferase sequence (described here), to give a bioluminescent read-out of protein levels. |
Michael Schrader
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December 2023
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