Jiewen Guan from Reading School, Reading and Paula Schrader from Exeter College performed a one-week work experience placement in our research group. They were introduced to mammalian cell culture techniques, cell transfection, protein expression, immunofluorescence and fluorescence—based microscopy and successfully applied those techniques to determine the subcellular localisation of an uncharacterised human protein of the ACBD family in cultured mammalian cells. The ACBD proteins, which can bind fatty acids and modulate cellular lipid metabolism, are of medical importance. Their great interest and enthusiasm was rewarded by a social event with the research team at the end of their work experience.
Congratulations to our Post-doctoral research associate Harley and his wife Rachael on the arrival of their baby Fflur! We look forward to seeing the latest member of the lab growing up!
Dr. Ruth Carmichael and Tina Schrader were featured in a video blog post from Promega, discussing research from the Schrader lab as well as how they have used Promega's Nano-Glo® Dual-Luciferase® Reporter (DLR) assay system together with the GloMax® Discover plate reader to measure endogenous expression of peroxisome proteins. Ruth and Tina used a CRISPR-Cas9 based strategy to endogenously tag peroxisomal proteins with the NanoLuc® luciferase sequence (described here), to give a bioluminescent read-out of protein levels.
A walk-through of the peroxisome from the lab of Prof. Maya Schuldiner.
For more information, check out mayaschuldiner.wixsite.com/schuldinerlab Huge congratulations to Beatriz Silva for successfully defending her Viva last week. After a hefty 4 hours, Dr Silva is ready to start an exciting post-doctoral research post in Luxembourg.
After many long years with the Schrader Lab, PhD student Suzan Kors successfully defended her PhD thesis with minor corrections. Suzan now moves to the EMBL and University of Heidelberg, Germany. We wish her all the best for the future!
Lab PhD students Beatriz Silva, Rechal Kumar and Suzan Kors worked with other PhD/PGR students on a handbook for organizing conferences, available on the Doctoral College Researcher Development website, for PGR across the UK. (Online since Sept 2022)
Suzan Kors, a PhD student in the Schrader lab, was awarded the Peroxisome Research Award at the biannual 8th Open European Peroxisome Meeting (OEPM), Sept. 22-24, 2022 in Aveiro, Portugal for the best paper published between Sept. 2020/2022.
The winning paper, Kors S, Hacker C, Bolton C, Maier R, Reimann L, Kitchener EJA, Warscheid B, Costello JL, Schrader M. (2022) Regulating peroxisome-ER contacts via the ACBD5-VAPB tether by FFAT motif phosphorylation and GSK3β in the Journal of Cell Biology, presents the first comprehensive molecular mechanism for the regulation of membrane contact sites between peroxisomes and the endoplasmic reticulum (ER) and has implications for the regulation of other organelle contacts. Suzan is a PhD student within the GW4 BIOMED MRC DTP, awarded to the Universities of Bath, Bristol, Cardiff, and Exeter from the Medical Research Council (MRC)/UKRI. Dr. Ruth Carmichael and Beatriz Silva from the Schrader lab were selected to give oral presentations about their work at the 8th Open European Peroxisome Meeting (OEPM), Sept. 22-24, 2022, in Aveiro, Portugal. Ruth, who is a research Co-I on an ongoing BBSRC project, presented recent data on an alternative pathway for peroxisome division (see below). Beatriz presented data on a new signalling and communication pathway between peroxisomes and the nucleus. She is a Marie Curie student within the EU H2020 MSC ITN program PERICO, which focusses on organelle interaction and communication.
Harley Worthy and Rechal Kumar also presented posters of their work Rechal Kumar, a PhD student from Michael Schrader’s group, is the winner of the ‘Draw your thesis’ competition” at the Postgraduate Life Sciences Society (PG-LSS) summer poster conference – 2022. Rechal is a Marie Curie student within the EU H2020 MSC ITN program PERICO, which focusses on organelle interaction and communication.
|
Michael Schrader
Archives
December 2023
Categories |